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DNA sequencing III : dealing with difficult templates / ed by Jan Kieleczawa.

Contributor(s): Publication details: Massachusetts: Jones and Bartlett Publishers, 2009.Description: xvi, 184 p., 8 p. of plates : ill. (some col.)ISBN:
  • 9780763742973 (alk. paper)
Other title:
  • DNA sequencing three
  • DNA sequencing 3
Subject(s): DDC classification:
  • 572.86 P9
Contents:
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
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Holdings
Item type Current library Call number Status Date due Barcode Item holds
Books Books Mahatma Gandhi University Library General Stacks 572.86 P9 (Browse shelf(Opens below)) Available 51883
Total holds: 0
Browsing Mahatma Gandhi University Library shelves, Shelving location: General Stacks Close shelf browser (Hides shelf browser)
572.82 Q31 Quantitative biology: 572.838 Q4 Gene duplication/ 572.86 P72 Recombinant DNA : 572.86 P9 DNA sequencing III : 572.86 Q11 Essential genetics : 572.86 Q12 The dynamic genome: 572.86 Q2 Codon evolution:

Includes bibliographical references and index.

Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.

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