DNA sequencing III : dealing with difficult templates / ed by Jan Kieleczawa.
Publication details: Massachusetts: Jones and Bartlett Publishers, 2009.Description: xvi, 184 p., 8 p. of plates : ill. (some col.)ISBN:- 9780763742973 (alk. paper)
- DNA sequencing three
- DNA sequencing 3
- 572.86 P9
Item type | Current library | Call number | Status | Date due | Barcode | Item holds |
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Books | Mahatma Gandhi University Library General Stacks | 572.86 P9 (Browse shelf(Opens below)) | Available | 51883 |
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572.82 Q31 Quantitative biology: | 572.838 Q4 Gene duplication/ | 572.86 P72 Recombinant DNA : | 572.86 P9 DNA sequencing III : | 572.86 Q11 Essential genetics : | 572.86 Q12 The dynamic genome: | 572.86 Q2 Codon evolution: |
Includes bibliographical references and index.
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
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